FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008061334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008061334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3748833
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATG41600611.096946703147353TruSeq Adapter, Index 15 (97% over 49bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA1043122.782519253324968Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA294620.7858979047612951Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC198310.5289912887557274Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG178900.477215176029447Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC160080.42701288641025087Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA156440.41730319808857846Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC153000.40812700912523975Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGAGATCGGAAGAGCACACGTCTGAACTCCAG151100.40305876522107015Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG123080.3283155051185262No Hit
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATG118020.31481797135268497TruSeq Adapter, Index 15 (100% over 36bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC111310.29691906788059114Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCA109370.2917441241047547Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACTCTCAACAACGGATATCTTGGCTCTCAGATCGGAAGAGCACACGTCT104860.279713713574331Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGTAGTAGGTTGTGTGGTAGATCGGAAGAGCACACGTCTGAACTCCA102850.27435204502307786Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATG87210.23263239520138665TruSeq Adapter, Index 15 (100% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATATCGTATG77340.20630420186762122TruSeq Adapter, Index 15 (97% over 41bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71040.18949897208011135No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC67060.17888233484927177Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC60240.1606900067300944No Hit
CGAGTGACGTAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA58610.1563419869596752Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC53710.14327125268050084Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC51860.1383363836159146Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGC47730.12731962186632478TruSeq Adapter, Index 15 (97% over 43bp)
CCGAGTGACGTAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC46890.12507892456132347Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTAAACTCCAGTCACATGTCAATCTCGTATG45020.12009070556090388TruSeq Adapter, Index 15 (97% over 39bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC42970.114622337137984Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGTGACGTAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT42900.11443561236256722TruSeq Adapter, Index 1 (100% over 35bp)
CAGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCA42800.1141688626834004TruSeq Adapter, Index 15 (100% over 39bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA42010.1120615402179825Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG40890.10907394381131409Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC38150.10176500260214312Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)