FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008061335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008061335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6818717
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG118271217.345081193426857No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATG3929785.7632249585955835TruSeq Adapter, Index 16 (97% over 49bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG1807272.650454623648408No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC981961.4400949621461046No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA867031.271544192257869Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT860591.2620996002620435No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG730791.0717412087933844No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC457320.6706833558277899Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC455700.668307542313312Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC354570.5199951838447028Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC269070.3946050261361485Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC243220.35669466851315285Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA241030.35348292061395126No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC232290.340665260048188Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA221030.3241518895710146Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA211680.3104396325584417Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC205350.30115636123335227Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC171980.2522175359382124Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC168670.24736325030060644Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG158690.23272706581018102Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCG151850.2226958531934967TruSeq Adapter, Index 7 (97% over 36bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC137380.201474852233932No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACG135210.19829243536577335No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTCAGATCGGAAGAGCACAC125480.18402288876338466No Hit
TGAGGTAGTAGATTGTATAGTTTAGATCGGAAGAGCACACGTCTGAACTC123080.18050316503823224Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA114390.1677588320500763Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGAGTGACGTAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC113550.16652692874627295Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGATCGGAAGAGC110570.16215660512087537No Hit
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCG107540.15771295391787046TruSeq Adapter, Index 7 (97% over 36bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC90780.13313354990388954Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC87990.12904187107339987Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGAGTGACGTAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC87620.12849924699910553Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC86880.12741399885051688Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGTGACGTAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC84360.12371828893910687TruSeq Adapter, Index 5 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82370.12079985135033466No Hit
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCA81420.11940662737579517Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTTGGTCCGAGTGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC81340.11928930325162343Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTAGACTGAAGCTCCTTGAGGAAGATCGGAAGAGCACACGTCTGAACTCC81290.1192159756740161Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTGTTCGGAAGATTTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC80350.11783741721499807Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC74000.10852481485886568Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGGTTGTGTGGAGATCGGAAGAGCACACGTCTGAACTCCAG73130.10724891500849794Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAAGATCGGAAGAGCACAC71830.10534239799070706No Hit
TGAGGTAGTAGGTTGTGTGGTAGATCGGAAGAGCACACGTCTGAACTCCA71270.10452112912150482Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCC71080.10424248432659694TruSeq Adapter, Index 16 (100% over 39bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT69530.10196932942076933Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC69410.10179334323451171No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)