FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008061337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008061337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6457791
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG6402029.913637651017197No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATG4208106.516314944227833TruSeq Adapter, Index 18 (100% over 49bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA1507192.333909536558244Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG1018341.576916936457064No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC594150.9200514541272705No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT575450.8910941837541662No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC468070.7248144140929924Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG388280.6012582321106397No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA333920.5170808408014443Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC313710.48578530955864013Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC274820.42556347828537655Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC267230.4138102332515871Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC256690.39748886267765554Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG251990.39021083215607316Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA227700.35259735101368256Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC199300.3086194644577379Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC191780.29697461562320615Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC178510.2764257932782278Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC166580.25795198389046653Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC164610.254901405139931Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTC162140.2510765678232696TruSeq Adapter, Index 1 (97% over 36bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC139500.21601813994909405Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGAGATCGGAAGAGCACACGTCTGAACTCCAG123370.19104055860587624Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA121420.18802095019798568No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA100800.1560905269309583Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTC99160.1535509588340657TruSeq Adapter, Index 1 (97% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACG96890.15003582494385465No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC95350.1476511085601872Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACACTCTGAGCGGTGGATCACTCGGCTCGCAGATCGGAAGAGCACACGTC88760.1374463806586494No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88420.13691988483368384No Hit
AGTTGGTCCGAGTGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC85530.1324446703214768Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCA83370.1290998733158134Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTCAGATCGGAAGAGCACAC78840.12208509070671379No Hit
TGAGGTAGTAGGTTGTGTGGTAGATCGGAAGAGCACACGTCTGAACTCCA76930.1191274229841133Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC72090.1116326000640157Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC69980.10836522891496489No Hit
TGAGGTAGTAGATTGTATAGTTTAGATCGGAAGAGCACACGTCTGAACTC69660.10786970343264438Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)