FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008061341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008061341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12394851
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATG194441915.687312417067378TruSeq Adapter, Index 22 (97% over 49bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG5125104.1348621294439125No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG1299771.0486370509818956No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC1202070.9698139977640715Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA1155780.9324678449139889Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT825810.6662524624136263No Hit
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCA753590.607986332389151Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC724030.584137719767668No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC546610.440997636841298Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG499470.402965715360354No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC452800.3653129835929452Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTAT442560.3570514885576277TruSeq Adapter, Index 22 (97% over 48bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG395090.3187533274905846No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA391170.31559072392237714Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC378230.3051509050007943Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA343630.27723608779161607Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC324020.2614150020843332Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGT295070.2380585293038214TruSeq Adapter, Index 12 (97% over 36bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTAT294880.2379052398451583TruSeq Adapter, Index 22 (97% over 48bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC292030.23560589796521153Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATG289410.2334921170089096TruSeq Adapter, Index 22 (97% over 39bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC261580.21103924524788562Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA246230.1986550705611548Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG243020.19606528549637264Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTAAACTCCAGTCACCGTACGATCTCGTATG240180.19377401148267132TruSeq Adapter, Index 22 (97% over 39bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT216950.17503235819454385Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC197700.15950171567209642Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACG196390.15844482519394545No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATATCGTATG191370.15439475633874097TruSeq Adapter, Index 22 (97% over 41bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC190560.15374125917286138Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGCGATCTCGTATG174270.1405987050590604TruSeq Adapter, Index 22 (97% over 39bp)
AACTCTCAACAACGGATATCTTGGCTCTCAGATCGGAAGAGCACACGTCT169740.13694396164988187Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC164390.1326276532085783Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAGTGTGACAATGGTGTTCAGATCGGAAGAGCACACGTCTGAACTCCA155390.1253665735876938Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTCAGATCGGAAGAGCACAC150480.12140525126118902No Hit
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC141150.11387793205420542Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCGGTCACCGTACGATCTCGTATG133650.107827032370135TruSeq Adapter, Index 22 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACCGTACGATCTCGTATGC125900.10157443602992887Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC125730.10143728230375662TruSeq Adapter, Index 22 (97% over 35bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATG125300.10109036405520325TruSeq Adapter, Index 22 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)