FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144
Sequences flagged as poor quality0
Sequence length105
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGTTCTCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTTTACAGAC1711.805555555555555No Hit
CACGACCAGAAAACTGGCCTAACGACGTTTGGTCAGTTCCATCAACATCA1711.805555555555555No Hit
TTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAA1510.416666666666668No Hit
TCGAAAAATTATCTTGATAAAGCAGGAATTACTACTGCTTGTTTACGAAT1510.416666666666668No Hit
TGCAATGCGACAGGCTCATGCTGATGGTTGGTTTATCGTTTTTGACACTC128.333333333333332No Hit
TAACAAAGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTG117.638888888888889No Hit
CTAAGTCAACCTCAGCACTAACCTTGCGAGTCATTTCTTTGATTTGGTCA85.555555555555555No Hit
CAGTGGATTAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCTCCCGAC74.861111111111112No Hit
TCGTCTAAGAAGTTTAAGATTGCTGAGGGTCAGTGGTATCGTTATGCGCC64.166666666666666No Hit
AAGCAAGCATCTCATTTTGTGCATATACCTGGTCTTTCGTATTCTGGCGT64.166666666666666No Hit
TTGCAAGAACGCGTACTTATTCGCCACCATGATTATGACCAGTGTTTCCA53.4722222222222223No Hit
TACATCTGTCAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGATATTG21.3888888888888888No Hit
AAGCAAGCATCTCACTTTGTGCATATACCTGGTCTTTCGTATTCTGGCGT10.6944444444444444No Hit
TGTGCCAGCACGATCCAATCTCGCATACCTCATCCAACCAGTAATGAGAA10.6944444444444444No Hit
GGGGATAGCACGAGAAAAGTGCGCAGACGAAGAACCGGCGAGAACAGGGG10.6944444444444444No Hit
TGTGACAGCACGATCCAATCTCGCATAAATCCTAATGGCAGTGCATTGAT10.6944444444444444No Hit
ATCTCGCATACTTTAAAGGTAGCTAAGGTAATGGTTCATATCATACAAAC10.6944444444444444No Hit
TGTGCAAGAAGGATCCAATCTCGCATAAAAAAAGACGGCGAGAACATGGT10.6944444444444444No Hit
ATCTCGCATATTTCATTGCGGCGAGAACATGGTGCGCAGGTTCTTGGTGA10.6944444444444444No Hit
ATCTCGCATAACTCCACTCGGCGAGAACATGGTGCGCAGGTTCTTGGTGA10.6944444444444444No Hit
TTGCTTGATTTGGTTGGACTTGGTTGCAAGTCTGCCGCTGATAAAGGAAA10.6944444444444444No Hit
TGGGAAAGAAAGAGAAAAGAGAGCAGAAGGTTGAGAAAGGAAAAAGAAGA10.6944444444444444No Hit
ACTGCATAGATGCCTTTGACTTATCTCGTATGCCGTCTTCTGCTTGAAAA10.6944444444444444Illumina PCR Primer Index 10 (96% over 28bp)
ATCTCGCATAGGATTAAGAATGTCCTTCCCCACCAGAAGCTTCTCTCCTT10.6944444444444444No Hit
TGTGCCAGCACGATCCAATCTCGCATAAATAGTTGCGGCGAGAACATGGT10.6944444444444444No Hit
TTGCTTGATTTGGTTGTACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAA10.6944444444444444No Hit
GGGGCCAGCACGATCCAATCTCGCATAGGAACTAGCGGCGAGAACATGGT10.6944444444444444No Hit
TGTGCAAGCACGATCCAATCTCGCATAAAAGTATTCAAAGGTGTGTGTCC10.6944444444444444No Hit
TGTGCCAGCACGATCCAATCTCGCATAGCAATAAACGGGGAGAACATGGT10.6944444444444444No Hit
ATCTCGCATACTTTCTATTACCCCTCAGACCTCCCGTACATGGGCTCCTA10.6944444444444444No Hit
GGAAAGGATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGC10.6944444444444444No Hit
TGTAATGAGACAGGCTCATGCTGATGGTTGGTTTATCGTTTTTGACACTC10.6944444444444444No Hit
TTGCAAGAACGCGTACTTATTCGCCACCATGATTATGACCAGTGATTCCA10.6944444444444444No Hit
TGTGCCAGCACGATCCAATCTCGCATAGCACAATGTGAAAGCAGCTTTCC10.6944444444444444No Hit
CTGGTTCTCACTTCTGTTATCCAGCTTCTTCGGCACCTGTTTTACAGACA10.6944444444444444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)