FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236299
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTG14570.61659169103551TruSeq Adapter, Index 10 (96% over 25bp)
TACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAAA5970.25264601204406284RNA PCR Primer, Index 31 (96% over 28bp)
TACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCT5020.21244271029500758RNA PCR Primer, Index 44 (95% over 24bp)
TACGACCCCCTCGCTCCAATTTCCCCTGTTATCTCGTATGCCGTCTTCTG4280.18112645419574352RNA PCR Primer, Index 25 (96% over 25bp)
TACGTGCCAGCACGATCCAATTTCCCCTGTTATCTCGTATGCCGTCTTCT2990.12653460234702646TruSeq Adapter, Index 25 (95% over 24bp)
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACA2910.123149061147106No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGC2590.10960689634742424No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGT2440.10325900659757341No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)