FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343006
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCTGC196955.7418820662029235RNA PCR Primer, Index 35 (96% over 27bp)
CGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCGTC174455.085916864428028No Hit
CGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTGCT119313.4783648099450155TruSeq Adapter, Index 10 (96% over 27bp)
CGTGCCAGCACGATCCTGAAATTCTGTTAACGAATCCTTCTCTTTATCTC54961.602304332868813No Hit
CGTGCCAGCACAGCTGCAGGTATAACTGGTTTGCCTTGTTATCTCGTATG16180.4717118650985697No Hit
CGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAATG12640.3685066733526527No Hit
CGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGTGT12410.3618012512900649No Hit
CGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAAAAA10570.30815787478936224Illumina PCR Primer Index 5 (96% over 27bp)
CGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACACA9170.2673422622344798No Hit
CGTGCCAGCATCAGAATCACTTATCTCGTATGCCGTCTTCTGCTTGAAAA8870.2585960595441479RNA PCR Primer, Index 10 (96% over 28bp)
CGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGCGA8650.2521821775712378No Hit
CGTGCCAGCACGATCCTATCTTGCACAGGTCTTCTGCCATTTCTTATCTC8540.24897523658478277No Hit
CGTGCCAGCACGATCCAATCTTGCATAGGTCTTCTGCCATTTCTTATCTC7920.23089975102476343No Hit
CGTGCCAGGCTAGGAGGAAGCTTATCTCGTATGCCGTCTTCTGCTTGAAA6090.17754791461373853Illumina PCR Primer Index 10 (100% over 29bp)
CGTGCCAGGCTGGGTATGCTAGAAACTTATCTCGTATGCCGTCTTCTGCT6030.17579867407567215Illumina PCR Primer Index 10 (96% over 27bp)
CGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTACCTCGTATGCCGTC5100.1486854457356431No Hit
CGTTCGCATAGAGACTCTGAAGAGAAGCCTGCTTATCTCGTATGCCGTCT4590.1338169011620788TruSeq Adapter, Index 10 (95% over 21bp)
CGTGCCAGCACGATCCAATCTCGCATACAGGGTGACCAGGACGTTCTTGA4380.12769455927884643No Hit
CGTGCCAGCCCATCTACCAGCTGTTCAGAACCTGATTATCTCGTATGCCG4240.12361299802335819No Hit
CGTGCCAGCACGATCCAATCTCTCATACAGGGTGACCAGGACGTTCTTGA3770.10991061380850481No Hit
CGTGCCAGCACGATCCAATCTCGCATACATTATCTCGTATGCCGTCTTCT3470.10116441111817287Illumina PCR Primer Index 6 (95% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)