FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21
Sequences flagged as poor quality0
Sequence length105
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCACGGAGTGCTTAATCCAACTTACCAAGCTGGGTTACGACGCGACG1257.14285714285714No Hit
TCAGTTTCTCATCTGAAAAATGCAATCAGTGAATGATCGACTGCATAGCA14.761904761904762No Hit
ACTCTCCTCCACATGTTATGCCTGAACAAGCTGGGCATTTGGCTCCCATC14.761904761904762No Hit
TCTGCAGTGATCACGCAGGTGATGTAACTCTTGCCTATGTCTGCCTACTG14.761904761904762No Hit
AGCACGATCCAATCTCGCATAGTCCTCCACCGCCTGTACATTGTTCTCTT14.761904761904762No Hit
AAAGTTCCCCAAATATATATTTTATACTGTACATAGATCGGAAGAGCACA14.761904761904762No Hit
AAAGTTCACAAACTATATATTATAAGATCGGAAGAGCACACGTCTGAACT14.761904761904762Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGCCAGCACGATCCAATCTCGCATACGTTAAAACGGCGAGAACATGGT14.761904761904762No Hit
AGTACCAGCACGATCCAATCTCGCATATAAAGAAGACTGTTGCTCCAAAC14.761904761904762No Hit
TTGTATCATGAGTTGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA14.761904761904762Illumina PCR Primer Index 10 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)