FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300134
Sequences flagged as poor quality0
Sequence length100
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCGT12420.41381516256072287No Hit
ACGTGCCAGCACGATCCTGAAATTCTGTTAACGAATCCTTCTCTTTATCT4420.14726755382595774No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACAC4110.1369388339874856No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAAT4010.13360698887830103No Hit
ACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCTG3160.10528630545023224RNA PCR Primer, Index 44 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)