FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332127
Sequences flagged as poor quality0
Sequence length100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCGT7570.22792486006858823No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACAC4850.14602847705847463No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAAT3770.1135107955691648No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGCT3520.10598355448367643No Hit
ACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTGC3350.10086503054554433RNA PCR Primer, Index 10 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)