FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250208
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCTG4730.18904271645990536RNA PCR Primer, Index 44 (96% over 25bp)
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAAT3650.14587862898068807No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACAC3410.136286609540862No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGCT2700.10791021869804322No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)