FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233711
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCG10950.4685273692723064No Hit
TACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTG3740.1600266996418654TruSeq Adapter, Index 10 (96% over 25bp)
TACGTGCCAGCACGATCCTGAAATTCTGTTAACGAATCCTTCTCTTTATC3730.1595988207658176No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACA3260.1394885135915725No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAA3160.13520972483109484No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGC2830.12108972192151844No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGT2710.11595517540894523No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)