FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518386
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTGC63111.2174325695524184RNA PCR Primer, Index 10 (96% over 26bp)
ACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCTG21820.4209218613156991RNA PCR Primer, Index 44 (96% over 25bp)
ACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAAAA12920.24923512594861746Illumina PCR Primer Index 5 (96% over 27bp)
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACAC7640.1473805233937645No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAAT7230.13947135918022477No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGCT5460.10532691855104112No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGTT5410.10436238632987774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)