FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425398
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTG8230.19346588371360468TruSeq Adapter, Index 10 (96% over 25bp)
TACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCT7080.16643237626881177RNA PCR Primer, Index 44 (95% over 24bp)
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACA6480.15232793760196334No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGT5660.13305187142393712No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAA5460.12835039186832098No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGC4870.11448102717925332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)