FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371523
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTGC36740.988902436726663RNA PCR Primer, Index 10 (96% over 26bp)
ACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCTG24360.6556794599526812RNA PCR Primer, Index 44 (96% over 25bp)
ACGACCCCCTCGCTCCAATTTCCCCTGTTATCTCGTATGCCGTCTTCTGC11830.3184190480804688TruSeq Adapter, Index 25 (96% over 26bp)
ACGTGCCAGCACGATCCAATTTCCCCTGTTATCTCGTATGCCGTCTTCTG7760.20886997574847319RNA PCR Primer, Index 25 (96% over 25bp)
ACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAAAA7330.1972959951335449Illumina PCR Primer Index 5 (96% over 27bp)
ACGTGCCAGCACGATCCTATCTTGCACAGGTCTTCTGCCATTTCTTATCT5810.15638331947147283No Hit
ACGTGCCAGCACGATCCAATCTTGCATAGGTCTTCTGCCATTTCTTATCT5720.15396085841253437No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACAC5220.1405027414184317No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAAT4100.11035655935164176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)