FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370799
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCTG16060.43311875166869385RNA PCR Primer, Index 44 (96% over 25bp)
ACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTGC14590.39347463180860787RNA PCR Primer, Index 10 (96% over 26bp)
ACGACCCCCTCGCTCCAATTTCCCCTGTTATCTCGTATGCCGTCTTCTGC5070.13673176033376572TruSeq Adapter, Index 25 (96% over 26bp)
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACAC4490.1210898627018951No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAAT3980.10733578030145713No Hit
ACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCGT3810.1027510861679778No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)