FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394221
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTGC42641.081626803239807RNA PCR Primer, Index 10 (96% over 26bp)
ACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCTG38810.9844731762133423RNA PCR Primer, Index 44 (96% over 25bp)
ACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAAAA7770.19709756710068718Illumina PCR Primer Index 5 (96% over 27bp)
ACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCGT7310.18542898526461046No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACAC6040.1532135528041378No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAAT5260.13342769664731205No Hit
ACGTGCCAGCACGATCCAATTTCCCCTGTTATCTCGTATGCCGTCTTCTG4380.11110519226525224RNA PCR Primer, Index 25 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)