FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514035
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCGT23730.46164171700370593No Hit
ACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTGC16950.3297440835740757RNA PCR Primer, Index 10 (96% over 26bp)
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACAC7930.15426965089925784No Hit
ACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCTG7510.14609900104078516RNA PCR Primer, Index 44 (96% over 25bp)
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAAT7130.13870650831169085No Hit
ACGTGCCAGCACGATCCTGAAATTCTGTTAACGAATCCTTCTCTTTATCT6370.12392152285350219No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGTT5360.10427305533669887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)