FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418484
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAA36440.8707620840940156Illumina PCR Primer Index 5 (96% over 27bp)
GTACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTC26570.6349107731717342RNA PCR Primer, Index 35 (95% over 24bp)
GTACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCT19340.46214431137152195TruSeq Adapter, Index 10 (95% over 24bp)
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGAC6060.14480840366656791No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGA5230.12497490943500827No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAG5020.11995679643666186No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAG4320.10322975310884047No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)