FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452084
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCC25250.5585245219914883No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGAC7580.16766795551269234No Hit
GTACGTGCCAGCACGATCCTGAAATTCTGTTAACGAATCCTTCTCTTTAT6450.14267260066713267No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGA6260.13846984188779077No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAG6120.13537307226090728No Hit
GTACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAA6040.13360348961697383Illumina PCR Primer Index 5 (96% over 27bp)
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAG5410.1196680262959981No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)