FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412503
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTG20150.4884812958936056TruSeq Adapter, Index 10 (96% over 25bp)
TACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCT11670.2829070334033934RNA PCR Primer, Index 44 (95% over 24bp)
TACGACCCCCTCGCTCCAATTTCCCCTGTTATCTCGTATGCCGTCTTCTG8020.19442282844003558RNA PCR Primer, Index 25 (96% over 25bp)
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACA5710.1384232357098009No Hit
TACGTGCCAGCACGATCCAATTTCCCCTGTTATCTCGTATGCCGTCTTCT5300.12848391405638263TruSeq Adapter, Index 25 (95% over 24bp)
TACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAAA5200.12605968926286598RNA PCR Primer, Index 31 (96% over 28bp)
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGC4640.11248403041917271No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAA4590.11127191802241439No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGT4380.10618104595602942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)