FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405346
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTC8090.19958257883388514RNA PCR Primer, Index 35 (95% over 24bp)
GTACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAA6200.15295574644871296Illumina PCR Primer Index 5 (96% over 27bp)
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGAC6010.14826839292851046No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAG4970.12261129997582312No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGA4850.11965086617358998No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAG4510.11126297040059603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)