FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395333
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCTGC18250.4616361396594769RNA PCR Primer, Index 35 (96% over 27bp)
CGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTGCT11370.2876056387905892TruSeq Adapter, Index 10 (96% over 27bp)
CGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACACT5270.13330534005509279No Hit
CGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAATC4410.11155152744648184No Hit
CGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGTTG4330.1095279169712622No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)