FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326911
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTGC5630.1722181266460902RNA PCR Primer, Index 10 (96% over 26bp)
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACAC4390.1342873136725286No Hit
ACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCGT4270.13061658983637747No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAAT3770.11532190718574781No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGTT3300.10094490549415591No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)