FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434229
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTC41100.9465051850521269RNA PCR Primer, Index 35 (95% over 24bp)
GTACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCT22160.5103298029380811TruSeq Adapter, Index 10 (95% over 24bp)
GTACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAA20410.4700284872728445Illumina PCR Primer Index 5 (96% over 27bp)
GTACGTGCCAGCACGATCCTATCTTGCACAGGTCTTCTGCCATTTCTTAT8220.1893010370104254No Hit
GTACGACCCCCTCGCTCCAATTTCCCCTGTTATCTCGTATGCCGTCTTCT7740.17824696185653194Illumina PCR Primer Index 12 (95% over 23bp)
GTACGTGCCAGCACGATCCAATCTTGCATAGGTCTTCTGCCATTTCTTAT7300.1681140596321296No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGAC6200.14278180407112376No Hit
GTACGTGCCAGAAAGCCGTTATCTCGTATGCCGTCTTCTGCTTGAAAAAA5240.12067365376333686Illumina PCR Primer Index 10 (96% over 27bp)
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGA5220.1202130672985913No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAG5120.1179101349748635No Hit
GTACGTGCCAGCACGATCCAATCCCGCATAAAGAGCTTGTCATTCACGAT4670.10754693951808839No Hit
GTACGTGCCTATAACCATGTTCTGTATTCTTATCTCGTATGCCGTCTTCT4450.10248048840588722TruSeq Adapter, Index 10 (95% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)