FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288051
Sequences flagged as poor quality0
Sequence length100
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCT4490.15587517488222571RNA PCR Primer, Index 44 (95% over 24bp)
TACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCG4090.14198874504861986No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACA3830.13296256565677605No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAA3420.11872897507733005No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGT3190.11074427792300669No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGC3040.1055368667354045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)