FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153
Sequences flagged as poor quality0
Sequence length105
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTTGACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAGTTTGTATC1912.418300653594772No Hit
CAGGGCGTTGAGTTCGATAATGGTGATATGTATGTTGACGGCCATAAGGC138.49673202614379No Hit
GTTCTTGCTGCCGAGGGTCGCAAGGCTAATGATTCACACGCCGACTGCTA117.18954248366013No Hit
CTCTAATCTCTGGGCATCTGGCTATGATGTTGATGGAACTGACCAAACGT106.535947712418301No Hit
GGCGCTACTGCAAAGGATATTTCTAATGTCGTCACTGATGCTGCTTCTGG85.228758169934641No Hit
TATCCATCTGAATGCAATGAAGAAAACCACCATTACCAGCATTAACCGTC74.57516339869281No Hit
GCGAGAAAGCTCAGTCTCAGGAGGAAGCGGAGCAGTCCAAATGTTTTTGA74.57516339869281No Hit
TATCCATCTGAATGCAATGAAGAATACCACCATTACCAGCATTAACCGTC31.9607843137254901No Hit
AGCACATACAAGGACCAGACCACACATGTGATTATCTCGTATGCCGTCTT21.3071895424836601No Hit
GCCAGCACGATCCAATCTCGCATAAGAACTTATGATCAAAGGCAGGAGAT10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAGGAAATCGGAAGAAGCCAGCTACACAA10.6535947712418301No Hit
TGCCAGCACACGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCGTCTT10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATATGTTGACCCAAGCAGATAACCTTCACT10.6535947712418301No Hit
TTTATTATGGCTTATAAAACAATCTCAGGAGGCGTCCTATTTTCAATGTT10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAAGAGTTACAGTTCCGCTATTGCTAAGC10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATATCTTAACATTGGAAGGGCTCGTTCTGC10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATACGTCCACGGTCAGAGACAGAACCTCCT10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAAAACTTCACTTGGAGCCGGCTCCCGCT10.6535947712418301No Hit
TATGTTGACGGCCATAAGGCTGCTCCTGACGTTCGTGATGAGTTTGTATC10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATATCTAGTCAGTAGATTTGGAAAGGCAAC10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATATCTGTTCCGGATGTAACTTACAGGTGA10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATACGATATCATGGCCTCATCGGCCCTCAG10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATACGAGCACGTGACTGATGATCTAAGTTT10.6535947712418301No Hit
TGCGCTACTGCAAAGGATATTTCTAATGTCGTCACTGATGCTGCTTCTGG10.6535947712418301No Hit
CCAGCACGATCCAATCTGCATACAACGAACAACCACGAAAACCCTCACTC10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATACATTGACAACCACTTTGGGTGTGGCGA10.6535947712418301No Hit
GTTCTTGCTGCCGAGGGTCGCAAGGCTAATGATTCACACGCCGTCTGCTA10.6535947712418301No Hit
CTCTAATCTCTGGGCATCTGGCTACGATGTTGATGGAACTGACCAAACGT10.6535947712418301No Hit
TTAACCATATGTTCGGTAGATAACTAAACAGTATGATCTGGTTGGCATTT10.6535947712418301No Hit
ACAGCACGATCCAATCTCGCATACTTTCTATCGGCGAGAACATGGTGCGC10.6535947712418301No Hit
TGCCAGCACGATCCAATCTCGCATAAGTACAGGACACATGAAGGTTACTT10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATATTTGCTGCCGGCGAGAACATGGTGCGC10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAATACCAGACAGGTCGCTGGGTGACTTC10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAGATTGTCACGGCGAGAACATGGTGCGC10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAAGTACTGTAACCACGAAAACCCTCACT10.6535947712418301No Hit
CCAGGAAGAAGAACAGAACTTTGTTTATCTCGTATGCCGTCTTCTGCTTG10.6535947712418301Illumina PCR Primer Index 10 (96% over 28bp)
CCAGCACGATCCAATCTCGCATATAATTACCCTTCTATACCTGGTATTCA10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAGGAACAAGAACCACGAAAACCCTCACT10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAGTTATAAATCGCCAAGGCCAAGTGAGA10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAATTTCTTATGCTGGGATTGAATTAGGC10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATATAAAATATTCTTCCACCTCATGGTTAT10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATATTTGGAATGACACAGAGGAACTCTGAA10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATACAACAAATCTTGGTCATTTCAATATCT10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATACCTGGACGGTAGCTAAGGTAATGGTTC10.6535947712418301No Hit
CCAGCACGATCCAGTCTCGCATACCAACAACCGGCGAGAACATGGTGCGC10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATATCAGGTACCGGCGAGAACATGGTGCGC10.6535947712418301No Hit
TGCCAGCACGATCCAATCTCGCATAACAAGACACGGCGAGAACATGGTGC10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAGAACGTGGAACCACGAAAACCCTCACT10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAAGTCGAACAACCACGAAAACCCTCACT10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATATGAAGATGAGTGGCTTTGCACAAAGCT10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAAAAACAAAAGCTTACCTTAGGACATGT10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAGATGTACTGAATCAGCTGGTTCCTGGC10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAATTAATAAAGAAAACAGGGATTTGGCG10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAACATTAGACCTCGCAGCCGCCGCTGTC10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAAGAATATTCGGCGAGAACATGGTGCGC10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAGATTGTATCTCCCAAGTAAACTTTCAC10.6535947712418301No Hit
TTGGCTAATCCAACAACCACTTCCAACCCTTTCTCCCTTCCCCTTCACTC10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAGGAACTTTCGGCGAGAACATGGTGCGC10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAGGTAGTATACTCCCACCCGGACCTCCA10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAGTATAATAAGAAAGCACTTCTTATGAA10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATATAAGATAGGGCTCTGCTGGTAACATCA10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATACTCGAGAACATGGTGCGCAGGTTCTTG10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATATATTAAACTGGCCCCTGTCATCTTCTG10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATACCTCAAGACGGCGAGAACATGGTGCGC10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATATGTGTAGTCAACGACAGCATCATTACT10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATACCTACAGAGGATGCACTTACTTCGAGG10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAGTACATTATGGGCGGCCAGGCTCTCGG10.6535947712418301No Hit
ACCACCCTTACCCTTGTAAAATCAAGTTATCTCGTATGCCGTCTTCTGCT10.6535947712418301Illumina PCR Primer Index 5 (96% over 26bp)
CCAGCACGATCCAATCTCGCATAAGATTTCAGTTTCCAGAAAACAGAAAT10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAATACCTTGCGGCGAGAACATGGTGCGC10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATAACACATGACTGAGTCTGCCTGGTTGAG10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATACCTTTAAACGACATACTTCGGGCATCA10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATACCAACACAGAACAGATGCTGAGCTTGG10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATACCTAGACTTGAACATATGTATCTTCTT10.6535947712418301No Hit
CCAGCACGATATCTCGCATAAATAGACAGAAGCAAGCCTGAAGATGTCCG10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATATATACAATCCCGATATCTTGTTTGTGG10.6535947712418301No Hit
TGCCAGCACGATCCAATCTCGCATATCTACTCCCATGCTTCCACATCGAA10.6535947712418301No Hit
CCAGCACGATCCAACCTCGCATATAATTTACCGTCACGCTTGAAGACCAC10.6535947712418301No Hit
CCAGCACGATCCAATCACGCATACCTGCACACCACCAGAGACCGCCTTAC10.6535947712418301No Hit
TGCCAGCACGATCCAATCTCGCATACTTCTATCAACCACGAAAACCCTCA10.6535947712418301No Hit
CCAGCACGATCCAATCTCGCATACCATCAGAACGTTACTTGCTAGTCTGT10.6535947712418301No Hit
TGCCAGCACGATCCATCTCGCATAGAACTACCGGTCTCTGCCCAGCTGGC10.6535947712418301No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)