FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312612
Sequences flagged as poor quality0
Sequence length100
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCG7230.23127711028367434No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACA4500.14394840889025373No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAA3780.12091666346781314No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGC3700.11835758064309752No Hit
TACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCT3440.11004056146277175RNA PCR Primer, Index 44 (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)