FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347510
Sequences flagged as poor quality0
Sequence length100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTC5660.16287301084860867RNA PCR Primer, Index 35 (95% over 24bp)
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGAC4710.13553566803832984No Hit
GTACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAA4140.11913326235216253Illumina PCR Primer Index 5 (96% over 27bp)
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGA4090.11769445483583206No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAG3930.11309027078357457No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAG3680.10589623320192225No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)