FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253547
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGA3690.14553514732968642No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGAC3620.14277431797654871No Hit
GTACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCC3240.12778695863094416No Hit
GTACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAA3230.12739255443763878Illumina PCR Primer Index 5 (96% over 27bp)
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAG3090.12187089573136342No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAG3060.12068768315144726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)