FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276188
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCGT8590.31102002983475024No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACAC4590.1661911451619911No Hit
ACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTGC4380.15858762871667126RNA PCR Primer, Index 10 (96% over 26bp)
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAAT3350.12129419091343578No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGCT2830.10246643590597708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)