FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252189
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTGC12890.5111245930631392RNA PCR Primer, Index 10 (96% over 26bp)
ACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCGT9080.36004742474889867No Hit
ACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAAAA6030.23910638449734128Illumina PCR Primer Index 5 (96% over 27bp)
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACAC4220.16733481634805641No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAAT3770.1494910563109414No Hit
ACGTGCCAGCACAGCTGCAGGTATAACTGGTTTGCCTTGTTATCTCGTAT3530.13997438429114673No Hit
ACGTGCCAGCACGATCCTGAAATTCTGTTAACGAATCCTTCTCTTTATCT2790.11063131223011313No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGCT2680.10626950422104058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)