FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255574
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCG16090.6295632576083638No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAA4330.1694225547199637No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACA4300.16824872639626878No Hit
TACGTGCCAGCACGATCCTGAAATTCTGTTAACGAATCCTTCTCTTTATC3830.14985874932504872No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGT3680.1439896077065742No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGC3380.13225132446962523No Hit
TACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTG3110.12168686955637115TruSeq Adapter, Index 10 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)