FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243579
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCGTC31701.3014258207809377No Hit
CGTGCCAGCACGATCCTGAAATTCTGTTAACGAATCCTTCTCTTTATCTC7340.30133960645211616No Hit
CGTGCCAGCACAGCTGCAGGTATAACTGGTTTGCCTTGTTATCTCGTATG5840.2397579430082232No Hit
CGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTGCT2970.12193169361890803TruSeq Adapter, Index 10 (96% over 27bp)
CGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACACT2840.11659461612043731No Hit
CGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGCTC2750.11289971631380374No Hit
CGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAATC2750.11289971631380374No Hit

[OK]Adapter Content

Adapter graph