FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254773
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCGT15730.6174123631625016No Hit
ACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTGC10780.42312175937010593RNA PCR Primer, Index 10 (96% over 26bp)
ACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCTG4720.18526295957577923RNA PCR Primer, Index 44 (96% over 25bp)
ACGTGCCAGCACGATCCTGAAATTCTGTTAACGAATCCTTCTCTTTATCT4490.17623531535916287No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACAC3890.1526849391419028No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAAT3650.14326478865499878No Hit
ACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAAAA2790.10950924941025933Illumina PCR Primer Index 5 (96% over 27bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)