FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260001
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCGTC15080.5799977692393491No Hit
CGTGCCAGCACGATCCTGAAATTCTGTTAACGAATCCTTCTCTTTATCTC6020.23153757100934225No Hit
CGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCTGC4680.17999930769497038RNA PCR Primer, Index 35 (96% over 27bp)
CGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACACT3240.1246149053272872No Hit
CGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTGCT3150.12115338017930699TruSeq Adapter, Index 10 (96% over 27bp)
CGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAATC2650.10192268491275033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)