FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321878
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAA10050.31223009960295517Illumina PCR Primer Index 5 (96% over 27bp)
GTACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTC6910.21467761077178313RNA PCR Primer, Index 35 (95% over 24bp)
GTACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCT5820.18081384872529344TruSeq Adapter, Index 10 (95% over 24bp)
GTACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCC5000.15533835801142046No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGAC4630.14384331951857537No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAG3840.11929985895277093No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAG3800.11805715208867956No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGA3450.10718346702788012No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)