FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307577
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTGC6590.214255292170741RNA PCR Primer, Index 10 (96% over 26bp)
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACAC4310.14012751278541632No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAAT3560.11574337482971744No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGCT3100.10078777021688878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)