FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310868
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTG10090.3245750607975089TruSeq Adapter, Index 10 (96% over 25bp)
TACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCT9430.3033441846700207RNA PCR Primer, Index 44 (95% over 24bp)
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACA4660.14990285265771966No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGT4530.14572101342048716No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAA4390.14121749424192906No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGC3990.1283502965889059No Hit
TACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCG3160.10165086145888289No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)