FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326501
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTG6950.2128630540182113TruSeq Adapter, Index 10 (96% over 25bp)
TACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCG5910.18101016535937103No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACA4740.14517566561817574No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGC4230.1295554990643214No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGT4120.1261864435330979No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAA3900.11944833247065094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)