FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335836
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCC11810.35165973868197575No Hit
GTACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAA6320.1881870913183816Illumina PCR Primer Index 5 (96% over 27bp)
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGAC5160.15364642265867864No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGA4400.13101632939887328No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAG4160.12386998415893473No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAG4090.12178563346395264No Hit
GTACGTGCCAGCACGATCCTGAAATTCTGTTAACGAATCCTTCTCTTTAT4060.12089234030896033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)