FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337497
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCC5170.15318654684337935No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAG4410.13066782815847253No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGAC4320.12800113778789146No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAG4180.12385295276698756No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGA3710.10992690305395307No Hit
GTACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAA3500.10370462552259724Illumina PCR Primer Index 5 (96% over 27bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)