FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326878
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTGC12740.38974785699863557RNA PCR Primer, Index 10 (96% over 26bp)
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACAC4960.15173856912976705No Hit
ACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAAAA4660.14256083309369244Illumina PCR Primer Index 5 (96% over 27bp)
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAAT4150.12695868183236558No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGTT3590.10982690789835964No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGCT3340.10217879453496412No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)