FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439368
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCC25350.5769650953187305No Hit
GTACGTGCCAGCACGATCCTGAAATTCTGTTAACGAATCCTTCTCTTTAT7120.1620509459041168No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGAC6870.15636095482602283No Hit
GTACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAA6670.15180896196354765Illumina PCR Primer Index 5 (96% over 27bp)
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGA6280.142932575881721No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAG5810.1322353926549043No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAG5430.12358660621620145No Hit
GTACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTC5320.1210830101418401RNA PCR Primer, Index 35 (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)