FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315471
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTTGGCCATCTCCTGATAGAATCTCTTATCTCGTATGCCGTCTTCTG77012.4411118613121334RNA PCR Primer, Index 27 (95% over 24bp)
TACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTG23500.7449179163853413TruSeq Adapter, Index 10 (96% over 25bp)
TACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCT11590.36738717663430237RNA PCR Primer, Index 44 (95% over 24bp)
ACTTTGGCCATCTCCTGATAGAATCTCTTATCTCGTATGCCGTCTTCTGC10240.32459401973557TruSeq Adapter, Index 10 (96% over 25bp)
TACGTGCCATCTCCTGATAGAATCTCTTATCTCGTATGCCGTCTTCTGCT7640.24217756941208543TruSeq Adapter, Index 10 (96% over 26bp)
TACGACCCCCTCGCTCCAATTTCCCCTGTTATCTCGTATGCCGTCTTCTG6450.20445619407172133RNA PCR Primer, Index 25 (96% over 25bp)
TACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAAA5870.18607098592263632RNA PCR Primer, Index 31 (96% over 28bp)
TACGTGCCAGCACGATCCAATTTCCCCTGTTATCTCGTATGCCGTCTTCT4970.15754221465681473TruSeq Adapter, Index 25 (95% over 24bp)
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACA4780.1515194740562524No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGT3880.12299070279043081No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGC3650.1157000168002764No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAA3630.11506604410548038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)