FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457957
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCT16740.3655365023353721RNA PCR Primer, Index 44 (95% over 24bp)
TACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTG8900.19434139013051444TruSeq Adapter, Index 10 (96% over 25bp)
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACA7310.15962197324202926No Hit
TCCTCCGCCACATTAAACTGCACACAGTTATCTCGTATGCCGTCTTCTGC6450.14084291756649642Illumina PCR Primer Index 10 (95% over 24bp)
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGT6090.13298191751627336No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAA5940.12970650082868043No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGC5380.11747827852833345No Hit
TACGACCCCCTCGCTCCAATTTCCCCTGTTATCTCGTATGCCGTCTTCTG4840.10568677845299887RNA PCR Primer, Index 25 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)