FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448382
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCT11160.24889491549616177RNA PCR Primer, Index 44 (95% over 24bp)
TACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTG11140.24844886726050555TruSeq Adapter, Index 10 (96% over 25bp)
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACA6910.15410966541921844No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAA5700.1271237471620181No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGC5660.1262316506907057No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGT4950.11039693832491046No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)