FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357874
Sequences flagged as poor quality0
Sequence length100
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTG58111.6237558470299605TruSeq Adapter, Index 10 (96% over 25bp)
TACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCT34490.9637470171065794RNA PCR Primer, Index 44 (95% over 24bp)
TACGACCCCCTCGCTCCAATTTCCCCTGTTATCTCGTATGCCGTCTTCTG18010.5032497471177007RNA PCR Primer, Index 25 (96% over 25bp)
TACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAAA17680.4940286245997194RNA PCR Primer, Index 31 (96% over 28bp)
TACGTGCCAGCACGATCCAATTTCCCCTGTTATCTCGTATGCCGTCTTCT13790.3853311500695776TruSeq Adapter, Index 25 (95% over 24bp)
TACGTGCCAGCACGATCCTATCTTGCACAGGTCTTCTGCCATTTCTTATC7940.22186579634172923No Hit
TACGTGCCAGCACGATCCAATCTTGCATAGGTCTTCTGCCATTTCTTATC7350.2053795469913992No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACA5300.1480968161978797No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAA5240.14642024846733767No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGT3900.10897690248523222No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGC3890.1086974745301419No Hit
TACGTGCCAGCACGATCCAATCTCCCCTGTTATCTCGTATGCCGTCTTCT3680.10282948747324476Illumina PCR Primer Index 12 (95% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)