FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377157
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTC32840.8707249235729417RNA PCR Primer, Index 35 (95% over 24bp)
GTACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAA30750.8153103349533484Illumina PCR Primer Index 5 (96% over 27bp)
GTACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCT13820.3664256529774073TruSeq Adapter, Index 10 (95% over 24bp)
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGAC5410.1434415906373208No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAG4890.12965422887550807No Hit
GTACGTGCCTATAACCATGTTCTGTATTCTTATCTCGTATGCCGTCTTCT4860.12885880415848042TruSeq Adapter, Index 10 (95% over 23bp)
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGA4590.1216999817052315No Hit
GTACGACCCCCTCGCTCCAATTTCCCCTGTTATCTCGTATGCCGTCTTCT4430.11745771654775067Illumina PCR Primer Index 12 (95% over 23bp)
GTAAGTGCCTATAACCATGTTCTGTATTCTTATCTCGTATGCCGTCTTCT4300.1140108761072975Illumina PCR Primer Index 10 (100% over 22bp)
GTACGTGCCAGCACGATCCAATCTTGCATAGGTCTTCTGCCATTTCTTAT4230.11215488510089963No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAG4160.11029889409450175No Hit
GTACGTGCCAGAAAGCCGTTATCTCGTATGCCGTCTTCTGCTTGAAAAAA3970.10526120421999327Illumina PCR Primer Index 10 (96% over 27bp)
GTACGTGCCAGCACGATCCTATCTTGCACAGGTCTTCTGCCATTTCTTAT3910.10367035478593796No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)