FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425782
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCT25790.6057090248061214RNA PCR Primer, Index 44 (95% over 24bp)
TACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTG21750.5108247882719327TruSeq Adapter, Index 10 (96% over 25bp)
TACGACCCCCTCGCTCCAATTTCCCCTGTTATCTCGTATGCCGTCTTCTG13910.3266930025224176RNA PCR Primer, Index 25 (96% over 25bp)
TACGTGCCAGCACGATCCAATTTCCCCTGTTATCTCGTATGCCGTCTTCT9570.22476290683965033TruSeq Adapter, Index 25 (95% over 24bp)
TACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAAA7530.17685106462931735RNA PCR Primer, Index 31 (96% over 28bp)
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACA6610.15524376324034364No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAA6220.14608414634719175No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGT5150.12095391538392887No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGC5080.1193098815825939No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)